Coding

Part:BBa_K368010:Design

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-24)


CMV-Promoter + TEV-Protease+p14*+TEV recogn. site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1493


Design Notes

-> "Cut'N'Survive" system


Source

(Knop et al.: Efficient protein depletion by genetically controlled deprotection of a dormant N-degron (2009)) CMV promoter from registry seems to be wrong....

References